PoplarV1, Main, Exploration, bibRecord, 002111

Phenolic compounds and expression of 4CL genes in silver birch clones and Pt4CL1a lines.

Identifieur interne : 002111 ( Main/Exploration ); précédent : 002110; suivant : 002112

Phenolic compounds and expression of 4CL genes in silver birch clones and Pt4CL1a lines.

Auteurs : Suvi Sutela [Finlande] ; Terhi Hahl [Finlande] ; Heidi Tiimonen [Finlande] ; Tuija Aronen [Finlande] ; Tiina Ylioja [Finlande] ; Tapio Laakso [Finlande] ; Pekka Saranp [Finlande] ; Vincent Chiang [États-Unis] ; Riitta Julkunen-Tiitto [Finlande] ; Hely H Ggman [Finlande]

Source :

PloS one [ 1932-6203 ] ; 2014.

RBID : pubmed:25502441

Descripteurs français

KwdFr :
- ARN messager (génétique), ARN messager (métabolisme), Betula (enzymologie), Betula (génétique), Betula (métabolisme), Clonage moléculaire (MeSH), Coenzyme A ligases (déficit), Coenzyme A ligases (génétique), Feuilles de plante (génétique), Phénols (composition chimique), Phénols (métabolisme), Phénomènes biomécaniques (MeSH), Populus (génétique), Régulation de l'expression des gènes végétaux (MeSH), Solubilité (MeSH), Végétaux génétiquement modifiés (MeSH).
MESH :
- composition chimique : Phénols.
- déficit : Coenzyme A ligases.
- enzymologie : Betula.
- génétique : ARN messager, Betula, Coenzyme A ligases, Feuilles de plante, Populus.
- métabolisme : ARN messager, Betula, Phénols.
- Clonage moléculaire, Phénomènes biomécaniques, Régulation de l'expression des gènes végétaux, Solubilité, Végétaux génétiquement modifiés.

English descriptors

KwdEn :
- Betula (enzymology), Betula (genetics), Betula (metabolism), Biomechanical Phenomena (MeSH), Cloning, Molecular (MeSH), Coenzyme A Ligases (deficiency), Coenzyme A Ligases (genetics), Gene Expression Regulation, Plant (MeSH), Phenols (chemistry), Phenols (metabolism), Plant Leaves (genetics), Plants, Genetically Modified (MeSH), Populus (genetics), RNA, Messenger (genetics), RNA, Messenger (metabolism), Solubility (MeSH).
MESH :
- chemical , chemistry : Phenols.
- chemical , deficiency : Coenzyme A Ligases.
- enzymology : Betula.
- genetics : Betula, Coenzyme A Ligases, Plant Leaves, Populus, RNA, Messenger.
- metabolism : Betula, Phenols, RNA, Messenger.
- Biomechanical Phenomena, Cloning, Molecular, Gene Expression Regulation, Plant, Plants, Genetically Modified, Solubility.

Abstract

A small multigene family encodes 4-coumarate:CoA ligases (4CLs) catalyzing the CoA ligation of hydroxycinnamic acids, a branch point step directing metabolites to a flavonoid or monolignol pathway. In the present study, we examined the effect of antisense Populus tremuloides 4CL (Pt4CL1) to the lignin and soluble phenolic compound composition of silver birch (Betula pendula) Pt4CL1a lines in comparison with non-transgenic silver birch clones. The endogenous expression of silver birch 4CL genes was recorded in the stems and leaves and also in leaves that were mechanically injured. In one of the transgenic Pt4CL1a lines, the ratio of syringyl (S) and guaiacyl (G) lignin units was increased. Moreover, the transcript levels of putative silver birch 4CL gene (Bp4CL1) were reduced and contents of cinnamic acid derivatives altered. In the other two Pt4CL1a lines changes were detected in the level of individual phenolic compounds. However, considerable variation was found in the transcript levels of silver birch 4CLs as well as in the concentration of phenolic compounds among the transgenic lines and non-transgenic clones. Wounding induced the expression of Bp4CL1 and Bp4CL2 in leaves in all clones and transgenic lines, whereas the transcript levels of Bp4CL3 and Bp4CL4 remained unchanged. Moreover, minor changes were detected in the concentrations of phenolic compounds caused by wounding. As an overall trend the wounding decreased the flavonoid content in silver birches and increased the content of soluble condensed tannins. The results indicate that by reducing the Bp4CL1 transcript levels lignin composition could be modified. However, the alterations found among the Pt4CL1a lines and the non-transgenic clones were within the natural variation of silver birches, as shown in the present study by the clonal differences in the transcripts levels of 4CL genes, soluble phenolic compounds and condensed tannins.

DOI: 10.1371/journal.pone.0114434
PubMed: 25502441
PubMed Central: PMC4263613

Affiliations:

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<term>Betula (genetics)</term>
<term>Betula (metabolism)</term>
<term>Biomechanical Phenomena (MeSH)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>Coenzyme A Ligases (deficiency)</term>
<term>Coenzyme A Ligases (genetics)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Phenols (chemistry)</term>
<term>Phenols (metabolism)</term>
<term>Plant Leaves (genetics)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Populus (genetics)</term>
<term>RNA, Messenger (genetics)</term>
<term>RNA, Messenger (metabolism)</term>
<term>Solubility (MeSH)</term>
</keywords>
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<term>ARN messager (métabolisme)</term>
<term>Betula (enzymologie)</term>
<term>Betula (génétique)</term>
<term>Betula (métabolisme)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Coenzyme A ligases (déficit)</term>
<term>Coenzyme A ligases (génétique)</term>
<term>Feuilles de plante (génétique)</term>
<term>Phénols (composition chimique)</term>
<term>Phénols (métabolisme)</term>
<term>Phénomènes biomécaniques (MeSH)</term>
<term>Populus (génétique)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Solubilité (MeSH)</term>
<term>Végétaux génétiquement modifiés (MeSH)</term>
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</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Betula</term>
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<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Betula</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Betula</term>
<term>Coenzyme A Ligases</term>
<term>Plant Leaves</term>
<term>Populus</term>
<term>RNA, Messenger</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term>
<term>Betula</term>
<term>Coenzyme A ligases</term>
<term>Feuilles de plante</term>
<term>Populus</term>
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<front><div type="abstract" xml:lang="en">A small multigene family encodes 4-coumarate:CoA ligases (4CLs) catalyzing the CoA ligation of hydroxycinnamic acids, a branch point step directing metabolites to a flavonoid or monolignol pathway. In the present study, we examined the effect of antisense Populus tremuloides 4CL (Pt4CL1) to the lignin and soluble phenolic compound composition of silver birch (Betula pendula) Pt4CL1a lines in comparison with non-transgenic silver birch clones. The endogenous expression of silver birch 4CL genes was recorded in the stems and leaves and also in leaves that were mechanically injured. In one of the transgenic Pt4CL1a lines, the ratio of syringyl (S) and guaiacyl (G) lignin units was increased. Moreover, the transcript levels of putative silver birch 4CL gene (Bp4CL1) were reduced and contents of cinnamic acid derivatives altered. In the other two Pt4CL1a lines changes were detected in the level of individual phenolic compounds. However, considerable variation was found in the transcript levels of silver birch 4CLs as well as in the concentration of phenolic compounds among the transgenic lines and non-transgenic clones. Wounding induced the expression of Bp4CL1 and Bp4CL2 in leaves in all clones and transgenic lines, whereas the transcript levels of Bp4CL3 and Bp4CL4 remained unchanged. Moreover, minor changes were detected in the concentrations of phenolic compounds caused by wounding. As an overall trend the wounding decreased the flavonoid content in silver birches and increased the content of soluble condensed tannins. The results indicate that by reducing the Bp4CL1 transcript levels lignin composition could be modified. However, the alterations found among the Pt4CL1a lines and the non-transgenic clones were within the natural variation of silver birches, as shown in the present study by the clonal differences in the transcripts levels of 4CL genes, soluble phenolic compounds and condensed tannins. </div>
</front>
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<DateCompleted><Year>2015</Year>
<Month>10</Month>
<Day>01</Day>
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<Month>03</Month>
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<ArticleTitle>Phenolic compounds and expression of 4CL genes in silver birch clones and Pt4CL1a lines.</ArticleTitle>
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<Abstract><AbstractText>A small multigene family encodes 4-coumarate:CoA ligases (4CLs) catalyzing the CoA ligation of hydroxycinnamic acids, a branch point step directing metabolites to a flavonoid or monolignol pathway. In the present study, we examined the effect of antisense Populus tremuloides 4CL (Pt4CL1) to the lignin and soluble phenolic compound composition of silver birch (Betula pendula) Pt4CL1a lines in comparison with non-transgenic silver birch clones. The endogenous expression of silver birch 4CL genes was recorded in the stems and leaves and also in leaves that were mechanically injured. In one of the transgenic Pt4CL1a lines, the ratio of syringyl (S) and guaiacyl (G) lignin units was increased. Moreover, the transcript levels of putative silver birch 4CL gene (Bp4CL1) were reduced and contents of cinnamic acid derivatives altered. In the other two Pt4CL1a lines changes were detected in the level of individual phenolic compounds. However, considerable variation was found in the transcript levels of silver birch 4CLs as well as in the concentration of phenolic compounds among the transgenic lines and non-transgenic clones. Wounding induced the expression of Bp4CL1 and Bp4CL2 in leaves in all clones and transgenic lines, whereas the transcript levels of Bp4CL3 and Bp4CL4 remained unchanged. Moreover, minor changes were detected in the concentrations of phenolic compounds caused by wounding. As an overall trend the wounding decreased the flavonoid content in silver birches and increased the content of soluble condensed tannins. The results indicate that by reducing the Bp4CL1 transcript levels lignin composition could be modified. However, the alterations found among the Pt4CL1a lines and the non-transgenic clones were within the natural variation of silver birches, as shown in the present study by the clonal differences in the transcripts levels of 4CL genes, soluble phenolic compounds and condensed tannins. </AbstractText>
</Abstract>
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</AffiliationInfo>
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<ForeName>Heidi</ForeName>
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<ArticleId IdType="doi">10.1371/journal.pone.0114434</ArticleId>
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</ArticleIdList>
<ReferenceList><Reference><Citation>Plant Physiol. 2009 Jun;150(2):924-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19395405</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biotechnol. 2001 Oct;19(2):201-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11725489</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Phytochemistry. 2005 Sep;66(17):2072-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16099486</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2010 Mar;185(4):893-908</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20015067</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Environ Entomol. 2008 Dec;37(6):1582-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19161703</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1991 May;3(5):435-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1840921</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Plant Biol. 2009 Sep 29;9:124</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19788757</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Physiol. 1996 Oct;37(7):957-65</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8979396</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2004 Feb 17;101(7):2209-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14769935</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 1999 Jul;19(1):9-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10417722</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2010 Oct;154(2):874-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20729393</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Biofuels. 2013 Apr 26;6(1):46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23622268</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2003 Aug;132(4):1790-800</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12913137</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Ann Bot. 2005 May;95(6):1025-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15760913</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Chem Ecol. 2001 Apr;27(4):779-89</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11446300</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Funct Integr Genomics. 2009 May;9(2):271-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18931865</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Evol. 1992 Jul;9(4):678-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1630306</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2008;179(4):987-1003</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18627494</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Planta. 2005 Nov;222(4):699-708</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15971066</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1991 May 5;266(13):8551-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2022667</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2011 Oct;157(2):574-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21807887</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Mol Biol. 2003 Feb;51(3):327-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12602864</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Evol. 1981;17(6):368-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7288891</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol Biochem. 2013 Apr;65:111-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23434928</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2012 Sep;24(9):3506-29</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23012438</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Bioinformatics. 2009 Oct 08;10 Suppl 11:S3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19811687</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Integr Plant Biol. 2010 Jan;52(1):98-111</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20074144</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2003 Nov;133(3):1051-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14612585</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2000 Nov 15;19(22):6150-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11080161</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Tree Physiol. 2000 May;20(9):607-613</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12651425</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Phytochemistry. 2011 Sep;72(13):1497-509</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21376356</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2009 Jan;149(1):370-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18971431</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2014 May 15;509(7500):376-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24670657</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Planta. 2006 Oct;224(5):1226-38</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16738863</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2012 Aug;40(15):e115</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22730293</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Theor Appl Genet. 2004 Aug;109(3):562-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15221141</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2010 Oct;154(2):887-98</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20639405</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Rep. 2013 Mar;40(3):2309-17</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23184048</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 1997 Jul;114(3):871-879</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12223748</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2002 Dec;32(5):701-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12472686</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2013 Sep 6;341(6150):1103-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23950498</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):5407-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9560289</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1997 Nov;9(11):1985-98</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9401123</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2013 Sep;75(5):781-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23663233</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Evol. 2013 Dec;30(12):2725-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24132122</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Phytochemistry. 2011 Sep;72(13):1551-65</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21354580</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4939-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12668766</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Bioinformatics. 2004 Aug 19;5:113</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15318951</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Biotechnol. 1999 Aug;17(8):808-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10429249</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Evol. 2013 May;30(5):1229-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23486614</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Evolution. 1985 Jul;39(4):783-791</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28561359</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8601-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12819348</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Chem Ecol. 2005 Apr;31(4):697-717</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16124246</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2011 May;62(8):2465-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21278228</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Planta. 2009 Oct;230(5):973-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19697057</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2002 Oct;130(2):796-807</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12376645</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Am Nat. 2010 Apr;175(4):481-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20170370</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2013 Jan 18;430(3):1151-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23246835</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2007 May 15;23(10):1289-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17379693</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2014 Mar;26(3):876-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24619612</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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Phenolic compounds and expression of 4CL genes in silver birch clones and Pt4CL1a lines.

Phenolic compounds and expression of 4CL genes in silver birch clones and Pt4CL1a lines.

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